By Shirley McBride, Mary Heenan, Martin Clynes (auth.), Dr. Martin Clynes (eds.)
Cell tradition innovations let quite a few molecular and phone organic inquiries to be addressed, delivering physiological stipulations when keeping off using laboratory animals. as well as simple thoughts, a variety of specialized useful protocols masking the next components are integrated: mobile proliferation and dying, in-vitro types for phone differentiation, in-vitro versions for toxicology and pharmacology, business program of animal mobilephone tradition, genetic manipulation and research of human and animal cells in culture.
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Extra resources for Animal Cell Culture Techniques
Mycoplasma orale colonies. The center of the colony is embedded in the agar, giving a "fried egg" type morphology (Kahane and Adoni 1993; Tully and Razin 1983). However, as long as the agars and broths are shown to support the growth of a range of common cell culture-infecting mycoplasma and the internal assay controls are as expected, any of these formulations are appropriate. The protocol outlined here is that used within this laboratory and which has been shown to support the growth of both M.
Similarly add 10 ~l of the second, third and fourth specimens to row A wells 7 -12, row E wells 1-6, and wells 7 -12, respectively. 8. Make seriall0-fold dilutions of each specimen from row A to row D and from row E to row H using 10 ~l volumes and a multichannel pipette. Fig. 4. Microtiter template 2 A B C D E F G H 3 4 5 6 7 8 9 10 11 12 2 Cell Culture and Diagnostic Virology 25 9. Add 25 fll of each different cell suspension to paired columns 1 and 7; 2 and 8; 3 and 9; 4 and 10; 5 and 11 and 6 and 12, respectively.
A/S Kemstrupvej 90 Roskilde DK 4000 Denmark Tel. 45 42 359065 Fax. 4542 350165 2 Cell Culture and Diagnostic Virology Bibby Sterilin Ltd, Tilling Drive Stone, Staffordshire STl50SA UK Tel. +44(0) 1785-812121 Fax. +44(0) 1785-813748 BDH Laboratory Supplies Poole Dorset UK BH151 TD Tel. (01202) 660-444 Fax. (01202) 666-856 Becton Dickinson European Division Chemin des Sources BP 37 Meylan CEDEX, France 76 416464 (phone) Hycor Biomedical Inc. 18800Von Karman Ave. Irvine, California 92715-1517, USA Tel.