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By Cole, James L

Analytical Ultracentrifugation, the most recent quantity in Methods in Enzymology, makes a speciality of analytical ultracentrifugation. The scope of this system has tremendously multiplied lately as a result of advances in instrumentation, algorithms and software program.

This quantity describes the most recent options within the box and within the purposes of analytical ultracentrifugation within the research of macromolecules, macromolecular assemblies, and biopharmaceuticals.

  • Timely contribution that describes a quickly altering field
  • Leading researchers within the field
  • Broad insurance: instrumentation, uncomplicated idea, facts research, and applications

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2010). Investigation of beta-carotene-gelatin composite particles with a multiwavelength UV/vis detector for the analytical ultracentrifuge. European Biophysics Journal with Biophysics Letters, 39(3), 397–403. , & Austin, B. (In Press). The CFA analytical ultracentrifuge architecture. Analytical ultracentrifugation: Instrumentation software and application. , & Kim, S. (1994). Analysis of protein-nucleic acid and proteinprotein interactions using multi-wavelength scans from the XL-A analytical ultracentrifuge.

Kim, S. (1994). Analysis of protein-nucleic acid and proteinprotein interactions using multi-wavelength scans from the XL-A analytical ultracentrifuge. In T. M. Schuster & T. M. ), Modern analytical ultracentrifugation: acquisition and interpretation of data for biological and synthetic polymer systems emerging biochemical and biophysical techniques: Part I (pp. 94–118). Boston: Birkha¨user. MacGregor, I. , Anderson, A. , & Laue, T. M. (2004). Fluorescence detection for the XLI analytical ultracentrifuge.

A first approximation of radial resolution is provided by an image of the upper cell edge. The ideal evaluation of radial resolution must take into account the light passing through the entire event space of a sample channel, which is typically 12 mm. The effects of alignment and focusing will be further complicated by the speed dependence of a sample meniscus formed at the window boundary, making the upper cell edge a simpler measure of radial resolution. The resolution determined at 280 nm for the open AUC design with 50 μm step size, as shown in SI 7 (http://dx.

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